Coding

Part:BBa_K3317005:Design

Designed by: erick hernandez   Group: iGEM19_UANL   (2019-10-16)


Vanillic acid -responsive transcriptional repressor (pcaU)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 94
    Illegal AgeI site found at 178
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pcaU is a functional Vanillic acid (DHBA) sensor from Acinetobacter sp. ADP1 adapted for E. coli


Source

Adapted by Jha, R. K. et.al 2014, enhanced by Meyer A. et.al 2018

References

Meyer, A. J., Segall-Shapiro, T. H., Glassey, E., Zhang, J., & Voigt, C. A. (2019). Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. Nature chemical biology, 15(2), 196.

Jha, R. K., Kern, T. L., Fox, D. T., & M. Strauss, C. E. (2014). Engineering an Acinetobacter regulon for biosensing and high-throughput enzyme screening in E. coli via flow cytometry. Nucleic acids research, 42(12), 8150-8160.